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. 1991 Oct;173(19):6058-65.
doi: 10.1128/jb.173.19.6058-6065.1991.

Genetic and sequence analyses of a Pseudomonas denitrificans DNA fragment containing two cob genes

B Cameron  1 , C Guilhot, F Blanche, L Cauchois, M C Rouyez, S Rigault, S Levy-Schil, J Crouzet
Affiliations

Genetic and sequence analyses of a Pseudomonas denitrificans DNA fragment containing two cob genes

B Cameron et al. J Bacteriol. 1991 Oct.

Abstract

A genetic analysis of a 12-kb DNA fragment containing Pseudomonas denitrificans cob genes was performed by transposon-mediated insertional mutagenesis. The nucleotide sequence and genetic analysis have shown that a 4.8-kb DNA subfragment carried two cob genes (cobS and cobT). Biochemical data concerning the complemented cobS and cobT mutants suggested that the cobS product was involved in cobalt insertion-mediating reactions and that the cobT product was involved in the transformation of precorrin-3 into cobyrinic acid.

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