doi: 10.1002/anie.200804735.
Semisynthesis of a homogeneous glycoprotein enzyme: ribonuclease C: part 2
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- PMID: 19180621
- DOI: 10.1002/anie.200804735
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Semisynthesis of a homogeneous glycoprotein enzyme: ribonuclease C: part 2
Angew Chem Int Ed Engl.
2009.
Abstract
Active RNase glycoprotein from three pieces: The glycoprotein enzyme ribonuclease C, which contains a complex saccharide N-glycan, was synthesized by sequential native chemical ligation. An optimized ligation and isolation protocol allowed the efficient assembly and refolding of the 124 amino acid enzyme.
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