Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009;48(11):1941-5.
doi: 10.1002/anie.200804735.

Semisynthesis of a homogeneous glycoprotein enzyme: ribonuclease C: part 2

Christian Piontek  1 , Daniel Varón Silva, Christian Heinlein, Claudia Pöhner, Stefano Mezzato, Petra Ring, Andreas Martin, Franz Xaver Schmid, Carlo Unverzagt
Affiliations

Semisynthesis of a homogeneous glycoprotein enzyme: ribonuclease C: part 2

Christian Piontek et al. Angew Chem Int Ed Engl. 2009.

Abstract

Active RNase glycoprotein from three pieces: The glycoprotein enzyme ribonuclease C, which contains a complex saccharide N-glycan, was synthesized by sequential native chemical ligation. An optimized ligation and isolation protocol allowed the efficient assembly and refolding of the 124 amino acid enzyme.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources