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. 2009 Feb 1:9:23.
doi: 10.1186/1471-2180-9-23.

Active immunization using exotoxin A confers protection against Pseudomonas aeruginosa infection in a mouse burn model

Ali Manafi  1 , Jamshid Kohanteb, Davood Mehrabani, Aziz Japoni, Masoud Amini, Mohsen Naghmachi, Ahmad Hosseinzadeh Zaghi, Nazanin Khalili
Affiliations

Active immunization using exotoxin A confers protection against Pseudomonas aeruginosa infection in a mouse burn model

Ali Manafi et al. BMC Microbiol. .

Abstract

Background: Pseudomonas aeruginosa is an important cause of nosocomial infection and may lead to septicemia and death. We evaluated the immunogenicity of semi-purified exotoxin A from the bacterium in a mouse burn model.

Methods: The toxoid was prepared from exotoxin A taken from toxigenic strains of P. aeruginosa (PA 103). 50 mice were immunized with the toxoid, burned with hot metal and infected with 1 x 10(8) CFU of toxigenic strains of P. aeruginosa (experimental group); 25 non-immunized mice were also burned and infected (control group). The mortality rate and presence of any exotoxin and P. aeruginosa in the sera, liver and spleen were determined.

Results: In the experimental group, 2 mice died before the burns were administered and were excluded from the study. The remainder (48 mice) were challenged with a lethal dose of P. aeruginosa and followed for 70 days. 3 of these mice died. Neither P. aeruginosa nor exotoxin A was not detected in the liver, spleen or sera of the surviving mice. The protective efficacy of toxoid vaccination was therefore 93.8%. In the control group, all mice died from bacteremia and septicemia, most (80%) within 6 days, and P. aeruginosa and exotoxin A were isolated from sera, spleen and liver.

Conclusion: Active immunization of mice using a semi-purified exotoxin A derived from P. aeruginosa was 93.8% effective at protecting mice from subsequent P. aeruginosa infections in a mouse burn model.

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