Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Mar;74(3):851-5.
doi: 10.1073/pnas.74.3.851.

Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis

Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis

R C Parker et al. Proc Natl Acad Sci U S A. 1977 Mar.

Abstract

The cleavage of DNA by restriction endonucleases can be limited by the addition of ethidium bromide. When closed circular DNA is used as a substrate, DNA with one-site cleavages of one or both strands can be made by adding appropriate amounts of dye. The singly cleaved DNA is a complete set of full-length permuted linear molecules. Fractionation of the products of a digestion of the permuted linears with a single-hitting restriction endonuclease by gel electrophoresis yields a series of bands that can be used to determine relative molecular weights of the DNA fragments in the gel without the introduction of standards. It is possible to determine the relative molecular weight of a fragment to within +/-2.5%. These molecular weights immediately allow the determination of the HindIII and Hpa I maps of simian virus 40. The HindIII map of bacteriophage PM2 was determined by this method with one ambiguity that was resolved by using traditional techniques.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4280-4 - PubMed
    1. Nucleic Acids Res. 1974 Jun;1(6):727-52 - PubMed
    1. J Mol Biol. 1975 Jan 25;91(3):315-28 - PubMed
    1. Biochemistry. 1975 Aug 26;14(17):3787-94 - PubMed
    1. Annu Rev Biochem. 1975;44:273-93 - PubMed

Publication types

LinkOut - more resources