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. 2009 Feb 1;34(3):362-4.
doi: 10.1364/ol.34.000362.

Fast-fluorescence dynamics in nonratiometric calcium indicators

M Gersbach  1 , D L Boiko, C Niclass, C C H Petersen, E Charbon
Affiliations

Fast-fluorescence dynamics in nonratiometric calcium indicators

M Gersbach et al. Opt Lett. .

Abstract

The fluorescence decay of high-affinity nonratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a model for accurate measurements of the free Ca2+ concentration and dissociation constants of nonratiometric calcium indicators.

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