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Comparative Study
. 2009 Mar;57(3):482-5.
doi: 10.1111/j.1532-5415.2008.02153.x. Epub 2009 Jan 23.

Respiratory syncytial virus outbreak in a long-term care facility detected using reverse transcriptase polymerase chain reaction: an argument for real-time detection methods

L Brett Caram  1 , Jodi Chen, E William Taggart, David R Hillyard, Rosemary She, Christopher R Polage, Jack Twersky, Kenneth Schmader, Cathy A Petti, Christopher W Woods
Affiliations
Comparative Study

Respiratory syncytial virus outbreak in a long-term care facility detected using reverse transcriptase polymerase chain reaction: an argument for real-time detection methods

L Brett Caram et al. J Am Geriatr Soc. 2009 Mar.

Abstract

Objectives: To report an outbreak of respiratory synctyial virus (RSV) in a long-term care facility (LTCF) during ongoing routine respiratory illness surveillance.

Design: Rapid antigen testing, viral culture, direct fluorescent antibody (DFA) testing, and reverse transcriptase polymerase chain reaction (RT-PCR) testing for up to 15 viruses in symptomatic residents and chart review.

Setting: A 120-bed LTCF.

Measurements: Comparison of rapid antigen testing, respiratory viral cultures, and DFA testing and RT-PCR in residents with symptoms of a respiratory tract infection.

Results: Twenty-two of 52 residents developed symptoms of a respiratory tract infection between January 29, 2008, and February 26, 2008. RSV was detected using RT-PCR in seven (32%) of the 22 cases. None of the seven cases had positive RSV rapid antigen testing, and only two had positive culture or DFA results. This outbreak occurred during a time when state wide RSV rates were rapidly declining. One patient was admitted to the hospital during the infection and subsequently died.

Conclusion: RSV may cause outbreaks in LTCFs that traditional diagnostic methods do not detect. RT-PCR can provide a more timely and accurate diagnosis of outbreaks, which allows for early symptomatic treatment, rational use of antibiotics, and improved infection control.

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Figures

Figure 1
Figure 1
Distribution of respiratory syncytial virus case patients in the two‐story long‐term care facility, North Carolina, January 29 through February 26, 2008.
Figure 2
Figure 2
Epidemic curve of patients with respiratory tract illness (RTI) during respiratory syncytial virus (RSV) outbreaks, long‐term care facility, North Carolina, January 29, 2008 through February 26, 2008. RSV infection occurred in 13% of residents during the outbreak period. Only one case of RTI was attributable to an alternate viral etiology.
See this image and copyright information in PMC

References

    1. Izurieta HS, Thompson WW, Kramarz P et al Influenza and the rates of hospitalization for respiratory disease among infants and young children. N Engl J Med 2000;342:232–239. - PubMed
    1. Falsey AR, Treanor JJ, Betts RF et al Viral respiratory infections in the institutionalized elderly: Clinical and epidemiologic findings. J Am Geriatr Soc 1992;40:115–119. - PMC - PubMed
    1. Fleming DM, Cross KW. Respiratory syncytial virus or influenza? Lancet 1993;342:1507–1510. - PubMed
    1. Falsey AR, Hennessey PA, Formica MA et al Respiratory syncytial virus infection in elderly and high‐risk adults. N Engl J Med 2005;352:1749–1759. - PubMed
    1. Thompson WW, Shay DK, Weintraub E et al Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA 2003;289:179–186. - PubMed

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