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. 2009 Apr;53(4):1628-9.
doi: 10.1128/AAC.01624-08. Epub 2009 Feb 2.

Breakpoints for susceptibility testing should not divide wild-type distributions of important target species

Maiken Cavling Arendrup  1 , Gunnar Kahlmeter, Juan Luis Rodriguez-Tudela, J Peter Donnelly
Affiliations

Breakpoints for susceptibility testing should not divide wild-type distributions of important target species

Maiken Cavling Arendrup et al. Antimicrob Agents Chemother. 2009 Apr.

Abstract

The fluconazole MIC distributions for Candida glabrata from testing 34 different clinical isolates and performing 51 tests on a single isolate mirrored each other. Since what is perceived as biological variation in isolates without resistance mechanisms is mainly methodological variation, breakpoints which divide this distribution not only lack a sound biological basis but also result in poor reproducibility of susceptibility characterization. This makes 2, 4, 8, and possibly 16 microg/ml unsuitable breakpoints for C. glabrata and fluconazole.

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Figures

FIG. 1.
FIG. 1.
Fluconazole MIC distributions of 34 individual C. glabrata isolates (black bars) compared with the MICs obtained by 51 repeated tests of a single C. glabrata isolate (striped bars) originally determined to have a MIC of 2 μg/ml.
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References

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