Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2009 Feb 1;65(Pt 2):169-72.
doi: 10.1107/S1744309109000153. Epub 2009 Jan 31.

Crystallization and preliminary crystallographic analysis of N-acetyltransferase Mpr1 from Saccharomyces cerevisiae

Takao Hibi  1 , Hiromi Yamamoto, Genichi Nakamura, Hiroshi Takagi
Affiliations
Comparative Study

Crystallization and preliminary crystallographic analysis of N-acetyltransferase Mpr1 from Saccharomyces cerevisiae

Takao Hibi et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

Mpr1 is an enzyme that catalyzes the N-acetylation of the toxic L-azetidine-2-carboxylic acid (AZC). Recently, Mpr1 has been shown to reduce levels of intracellular reactive oxygen species (ROS) under oxidative stress. The natural substrate involved in the ROS elimination in vivo is still unknown. Mpr1 has been purified and crystallized in space groups P1 and P3(1)12. X-ray data were collected to 1.9 A resolution from a trigonal crystal soaked with AZC.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Crystals of Mpr1 from yeast. (a) A single triclinic crystal (form I) obtained by the sitting-drop method. (b) A prism-shaped trigonal crystal (form II) obtained in the presence of MgCl2.
Figure 2
Figure 2
Diffraction image obtained on Photon Factory beamline BL-5A. Diffraction data were collected at a wavelength of 0.9793 Å with a crystal-to-detector distance of 237.8 mm. The resolution at the edges of the image is 1.9 Å.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Ambrosone, C. B. & Kadlubar, F. F. (1998). Biomarkers: Medical and Workplace Applications, edited by M. L. Mendelsohn, L. C. Mohr & J. P. Peeters, pp. 189–210. Washington DC: Joseph Henry Press.
    1. Bradford, M. M. (1976). Anal. Biochem.72, 248–254. - PubMed
    1. Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760–763. - PubMed
    1. Dalhoff, K., Jensen, K. B. & Poulsen, H. E. (2005). Methods Enzymol.400, 618–627. - PubMed
    1. Du, X. & Takagi, H. (2005). J. Biochem.138, 391–397. - PubMed

Publication types

MeSH terms

Substances