DNA-directed self-assembly of fluorescent dye-labeled streptavidin arrays for protein detection

Abstract

In this work, a DNA lattice was utilized to direct protein self-assembly. A model biosensor system was fabricated by self-assembly of rhodamine dye-labeled streptavidin molecules on a two-tile DNA system. This self-assembly nanofabrication technique has numerous possible biotechnology applications, including use in multiplex biosensing systems that have binding of several biological molecules encoded in a multiple-tile DNA lattice.