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. 2009 Feb 6;4(2):103-6.
doi: 10.1016/j.stem.2008.12.001.

Optimal timing of inner cell mass isolation increases the efficiency of human embryonic stem cell derivation and allows generation of sibling cell lines

Alice E Chen  1 , Dieter Egli, Kathy Niakan, Jie Deng, Hidenori Akutsu, Mariko Yamaki, Chad Cowan, Claire Fitz-Gerald, Kun Zhang, Douglas A Melton, Kevin Eggan
Affiliations

Optimal timing of inner cell mass isolation increases the efficiency of human embryonic stem cell derivation and allows generation of sibling cell lines

Alice E Chen et al. Cell Stem Cell. .
No abstract available

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Conflict of interest statement

COMPETING INTEREST STATEMENT

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1. OCT4 and CDX2 Expression During Human Preimplantation Development and Gene Expression Analysis of hESC Lines
Representative human preimplantation embryos on day 5 (A), day 6 (B) and day 8 (C) stained for the TE marker, CDX2, and ICM marker, OCT4. Total cell numbers for each day indicated. Bar indicates relative size for comparison. (D) Cluster analysis of 16 hESC lines derived on different days of development. Sibling cell lines marked with identical colors. (E, F) Pair-wise comparison of global gene expression profiles between different cell lines. Comparison of 16 cell lines yields 16x15/2=120 data points for all possible pair-wise comparisons. The higher the Pearson correlation coefficient (R), the more similar the gene expression profile between two lines. Pairs were ordered from high to low according to the Pearson correlation coefficient (ranked order). (E) Pairwise comparisons of hESC lines derived on the same versus different days. (F) Pairwise comparisons of sibling hESC lines versus unrelated lines.
See this image and copyright information in PMC

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