[Carbonyl groups content in serum proteins as an indicator of disease activity in children with inflammatory bowel diseases and its dependence on the other markers of inflammatory process]

Abstract

In patients with inflammatory bowel diseases, in inflamed alimentary tract mucosa the number of phagocytic cells (macrophages, neutrophils, monocytes), which are the source of reactive oxygen forms, is increased as compared with healthy mucosa. The intensity of the inflammation correlates with the production of free oxygen radicals.

The aim of the study: To demonstration of connection between inflammatory process activity in ulcerative colitis and Crohn's disease and carbonyl groups level.

Material and methods: The study comprised 63 children hospitalized in The 2nd Chair and Department of Pediatrics, Gastroenterology and Feeding of Children. In 50 children inflammatory bowel diseases were diagnosed, including 26 with ulcerative colitis, 17 with Crohn's disease and 7 with indeterminate colitis; 13 patients composed control group. In all patients the amount of carbonyl groups in serum proteins, erythrocytes sedimentation rate, C-reactive protein, seromucoid, cytoplasmic and perinuclear types of antinuclear antibodies against neutrophils cytoplasm, were determined.

Results: Among the children with inflammatory bowel diseases 58% had an increased content of carbonyl groups in the serum as compared to 7.7% in control group. In patient with inflammatory bowel diseases an improper concentration of carbonyl groups was more frequently observed in ulcerative colitis (69.2%) and this frequency increased along with activity of the disease.

Conclusions: The content of carbonyl groups in the serum in patients with inflammatory bowel diseases increases with activity of inflammatory process and other selected markers of inflammation. There is a need to answer the question whether free radical scavengers can influence the treatment course in patients with inflammatory bowel diseases and whether they should be used routinely.