Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Nov;65(11):6331-3.
doi: 10.1128/JVI.65.11.6331-6333.1991.

The 5' end of coronavirus minus-strand RNAs contains a short poly(U) tract

M A Hofmann  1 , D A Brian
Affiliations

The 5' end of coronavirus minus-strand RNAs contains a short poly(U) tract

M A Hofmann et al. J Virol. 1991 Nov.

Abstract

A radiolabeled oligodeoxynucleotide primer that anneals near the common 5' end of bovine coronavirus minus-strand RNAs was extended with reverse transcriptase, and a major product suggesting poly(U) tracts of 8 to 20 nucleotides was found. The extended primer molecules were ligated head to tail, amplified by the polymerase chain reaction, cloned, and sequenced, and poly(U) tracts of 9 to 26 nucleotides were found. Poly(A) tails of 100 to 130 nucleotides on the 3' end of coronavirus plus-strand mRNAs and genome must, therefore, be generated by a mechanism that uses only a short poly(U) template. This pattern contrasts with that of other cytoplasmic, polyadenylated, plus-strand animal RNA viruses which utilize a full-length poly(U) template for poly(A) synthesis.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1974 Aug;71(8):2983-7 - PubMed
    1. PCR Methods Appl. 1991 Aug;1(1):43-5 - PubMed
    1. J Virol. 1976 Nov;20(2):446-64 - PubMed
    1. J Biochem. 1977 Oct;82(4):1103-8 - PubMed
    1. Virology. 1978 May 15;86(2):494-506 - PubMed

Publication types

LinkOut - more resources