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. 2009 Jun;23(6):1182-6.
doi: 10.1038/leu.2009.16. Epub 2009 Feb 12.

The CLLU1 expression level is a stable and inherent feature of the chronic lymphocytic leukemia clone

A M Buhl, G W Novotny, P Josefsson, J E Nielsen, L B Pedersen, C Geisler, L Z Rassenti, T J Kipps, J Jurlander, H Leffers

The CLLU1 expression level is a stable and inherent feature of the chronic lymphocytic leukemia clone

A M Buhl et al. Leukemia. 2009 Jun.
No abstract available

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Figures

Figure 1
Figure 1
Expression of the CLLU1 splice variants. (a) Overview of the location of primers used for the CLLU1 splice variant-specific QRT-PCR reactions. (b) The expression patterns of the CLLU1 splice variants were investigated in 25 chronic lymphocytic leukemia (CLL) patients. ΔCt is the number of PCR cycles required to reach the threshold level for CLLU1 after normalization to the β2-M internal standard. ■ — X: cDNA1, Y: cDNA2/4, r = 0.90, P < 0.0001, Δ — X: cDNA1, Y: cDNA3, r = 0.90, P < 0.0001, ● — X: cDNA2/4, Y: cDNA3, r = 0.91, P < 0.0001. (c) Blood or marrow samples were collected from the same untreated patient at the same time point (n = 15). CLLU1 expression was determined by QRT-PCR (cDNA1 splice variant). Using linear regression analysis we found β = 0.89 ± 0.05, r = 0.98, P < 0.0001. The dotted lines show the 95% confidence intervals.
Figure 2
Figure 2
In situ hybridization (ISH) using probes complementary to Exon 3 or CDS sequences of CLLU1. (a) Overview of the CLLU1 gene on 12q22. The two ISH probes are shown in blue. Left panels (b and d) show ISH to sample UPN73, a CLL patient with a CLLU1 expression level of 1034-fold above B-cell levels. The right panels (c and e) show ISH to purified normal B-cell controls. The insets show hybridization with sense probe controls. The bars correspond to 10 µm. (f) Enlargement of ISH-stained chronic lymphocytic leukemia (CLL) cells from UPN73 (exon 3 probe). (g) Giemsa-stained CLL cells.
Figure 3
Figure 3
CLLU1 expression is a stable feature of the particular chronic lymphocytic leukemia (CLL) clone. (a) In all, 21 untreated CLL patients were followed longitudinally to determine whether CLLU1 expression changed over time. RQ(cDNA1) indicates the CLLU1 expression level (cDNA1 splice variant) relative to the normal B-pool calibrator. The biclonal patient is marked with closed circles and a dotted line. (b) Cell samples were obtained from 11 CLL patients before treatment and after relapse. White blood cell (WBC) counts in the patient before treatment (pre-Tx), after treatment (post-Tx) and after relapse (post-relapse). (c) CLLU1 expression RQ(cDNA1) measured in CLL samples pre-Tx and post-relapse.
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References

    1. Buhl AM, Jurlander J, Jorgensen FS, Ottesen AM, Cowland JB, Gjerdrum LM, et al. Identification of a gene on chromosome 12q22 uniquely overexpressed in chronic lymphocytic leukemia. Blood. 2006;107:2904–2911. - PubMed
    1. Oppliger Leibundgut E, Dissler D, de Beer D, Marti D, Schuller JC, Duerig J, et al. CLLU1 expression distinguishes B-cell chronic lymphocytic leukemia from other B-cell lymphoproliferative disorders. Haematologica. 2007;92(Suppl 1):0522.
    1. Buhl AM, Jurlander J, Geisler CH, Pedersen LB, Andersen MK, Josefsson P, et al. CLLU1 expression levels predict time to initiation of therapy and overall survival in chronic lymphocytic leukemia. Eur J Haematol. 2006;76:455–464. - PubMed
    1. Josefsson P, Geisler CH, Leffers H, Petersen JH, Andersen MK, Jurlander J, et al. CLLU1 expression analysis adds prognostic information to risk prediction in chronic lymphocytic leukemia. Blood. 2007;109:4973–4979. - PubMed
    1. Hayette S, Hayette S, Bastard C, Mozziconacci MJ, Messana C, Charlot C, et al. Expression levels of CLLU1 and LPL: new disease-assessment tools in CLL patients in Binet stage A? Haematologica. 2007;92(Suppl 1):0908.

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