Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977;53(1-2):101-8.
doi: 10.1007/BF01314851.

Expression of the human cytomegalovirus genome in mouse cells and in human-mouse heterokaryons

I Boldogh, E Gönczöl, L Gärtner, G Váczi

Expression of the human cytomegalovirus genome in mouse cells and in human-mouse heterokaryons

I Boldogh et al. Arch Virol. 1977.

Abstract

Mouse cells with an established human cytomegalovirus (HCMV) infection were fused with susceptible human embryonic fibroblast cells. CMV-specific early antigens could be demonstrated in the cytoplasm and cell-membrane of the heterokaryons. Treatment with 5-iodo-2'-deoxyuridine (IUdR) of the heterokaryons or of the latently infected mouse cells, prior to their fusion with human cells, could induce the appearance of immunoflourescent elements, characterised as late antigens, and of infectious virus. Our data show that the mouse cells, in the latent stage of infection, contain the whole virus genome and that the replication of the virus is controlled by a genetic mechanism of the host cells both in virus-harbouring mouse cells and in heterokaryons.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1972 Jan;69(1):83-5 - PubMed
    1. J Virol. 1975 Feb;15(2):297-304 - PubMed
    1. Adv Cancer Res. 1974;19(0):265-302 - PubMed
    1. Virology. 1964 Oct;24:229-32 - PubMed
    1. Arch Gesamte Virusforsch. 1972;38(4):297-305 - PubMed