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. 2009 Feb 16:6:24.
doi: 10.1186/1743-422X-6-24.

Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China

Wen Zhang  1 , Shixing Yang, Quan Shen, Junfeng Liu, Tongling Shan, Fen Huang, Huibo Ning, Yanjun Kang, Zhibiao Yang, Li Cui, Jianguo Zhu, Xiuguo Hua
Affiliations

Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China

Wen Zhang et al. Virol J. .

Abstract

In the present study, a genotype 4 HEV strain was identified in the fecal specimen from a seven months old infant with no symptom of hepatitis in Shanghai Children's hospital. The full capsid protein gene (ORF2) sequence of this strain was determined by RT-PCR method. Sequence analysis based on the full ORF2 sequence indicated that this HEV strain shared the highest sequence identity (97.6%) with another human HEV strain isolated from a Japanese patient who was infected by genotype 4 HEV during traveling in Shanghai. Phylogenetic analysis showed that this genotype 4 HEV was phylogenetically far from the genotype 4 HEV strain that was commonly prevalent in Shanghai swine group, suggesting that this strain may not come from swine group and not involved in zoonotic transmission in this area.

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Figures

Figure 1
Figure 1
Phylogenetic analysis based on the complete ORF2 sequence of the isolate in this study and other 10 genotype 4 HEV isolates in China, using the neighbor-joining method and evaluated using the interior branch test method with Mega 4 software. Percent bootstrap support is indicated at each node. The scale bar represents nucleotide substitutions per base. GenBank accession no. and origin are indicated. A genotype 1 HEV strain was included as outgroup.
Figure 2
Figure 2
Sequence analysis based on the complete ORF2 sequence of the isolate in this study and other 10 genotype 4 HEV isolates in China, using DNAstar software package.
See this image and copyright information in PMC

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