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. 2009 Feb:Chapter 4:4.9.1-4.9.13.
doi: 10.1002/0471142735.im0409s84.

Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation

Christopher R Parish  1 , Megan H Glidden  1 , Ben J C Quah  1 , Hilary S Warren  1
Affiliations

Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation

Christopher R Parish et al. Curr Protoc Immunol. 2009 Feb.

Abstract

The stable incorporation of the intracellular fluorescent dye 5-(and -6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) into cells provides a powerful tool to monitor cell migration, and to quantify cell division, because of the sequential decrease in fluorescent labeling in daughter cells. CFSE-labeled lymphocytes have been used to analyze the relationship between cell division and differentiation of cell function, and cell proliferation versus apoptosis, both in vivo and in vitro, and have allowed analysis of the site of response to antigens in vivo.

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References

Literature Cited

References
    1. Ford, W.L. 1978. The preparation and labeling of lymphocytes. In Handbook of Experimental Immunology, Vol. 3 (D.M. Weir, ed.) p. 23.1. Blackwell Scientific, Oxford.
    1. Garton, H.J. and Schoenwolf, G.C. 1996. Improving the efficacy of fluorescent labeling for histological tracking of cells in early mammalian and avian embryos. Anat. Rec. 244:112-117.
    1. Gett, A.V. and Hodgkin, P.D. 2000. A cellular calculus for signal integration by T cells. Nat. Immunol. 1:239-244.
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Key References
    1. Gett and Hodgkin, 2000. See above.
    1. Lyons and Parish, 1994. See above.
    1. Parish, 1999. See above.
    1. Hawkins et al., 2007. See above.
Internet Resources
    1. http://www.vsh.com/
    1. http://www.flowjo.com/

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