doi: 10.1016/0923-2508(91)90018-6.
The complex of oriC DNA with the DnaA initiator protein
Affiliations
- PMID: 1925008
- DOI: 10.1016/0923-2508(91)90018-6
Item in Clipboard
The complex of oriC DNA with the DnaA initiator protein
Res Microbiol.
1991 Feb-Apr.
Free article
Abstract
We describe several experimental approaches relating to the early steps in the initiation of DNA replication at oriC. 1) A matrix is given which enables calculatation of the relative affinity of DnaA boxes for DnaA protein; 2) base changes within single Dna A boxes in oriC have little effect on oriC function; 3) mutations which change the distance between DnaA boxes inactivate oriC, but changes by one helical turn (+ and -) result in near wild-type oriC activity; 4) a Fis binding site was located at oriC coordinates 206-220; 5) KMnO4 probing demonstrates Dna-A-dependent unwinding in the left part of oriC in vivo and in vitro.
Similar articles
-
The complex for replication initiation of Escherichia coli.Chromosoma. 1992;102(1 Suppl):S1-6. doi: 10.1007/BF02451779. Chromosoma. 1992. PMID: 1291230
-
A comprehensive set of DnaA-box mutations in the replication origin, oriC, of Escherichia coli.Mol Microbiol. 1996 Jul;21(2):301-11. doi: 10.1046/j.1365-2958.1996.6481362.x. Mol Microbiol. 1996. PMID: 8858585
-
Ordered and sequential binding of DnaA protein to oriC, the chromosomal origin of Escherichia coli.J Biol Chem. 1996 Jul 19;271(29):17035-40. doi: 10.1074/jbc.271.29.17035. J Biol Chem. 1996. PMID: 8663334
-
E. coli minichromosome replication: regulation of initiation at oriC.Res Microbiol. 1991 Feb-Apr;142(2-3):127-30. doi: 10.1016/0923-2508(91)90019-7. Res Microbiol. 1991. PMID: 1925009 Review.
-
IHF and Fis as Escherichia coli Cell Cycle Regulators: Activation of the Replication Origin oriC and the Regulatory Cycle of the DnaA Initiator.Int J Mol Sci. 2023 Jul 18;24(14):11572. doi: 10.3390/ijms241411572. Int J Mol Sci. 2023. PMID: 37511331 Free PMC article. Review.
Cited by
-
Anucleate cell production by Escherichia coli delta hns mutant lacking a histone-like protein, H-NS.J Bacteriol. 1995 Jun;177(12):3589-92. doi: 10.1128/jb.177.12.3589-3592.1995. J Bacteriol. 1995. PMID: 7768871 Free PMC article.
-
Mutations in the DnaA binding sites of the replication origin of Escherichia coli.Mol Gen Genet. 1992 May;233(1-2):81-8. doi: 10.1007/BF00587564. Mol Gen Genet. 1992. PMID: 1603077
-
Molecular flip-flops formed by overlapping Fis sites.Nucleic Acids Res. 2003 Nov 15;31(22):6663-73. doi: 10.1093/nar/gkg877. Nucleic Acids Res. 2003. PMID: 14602927 Free PMC article.
-
The structure of the initiation complex at the replication origin, oriC, of Escherichia coli.Nucleic Acids Res. 1993 Nov 11;21(22):5025-33. doi: 10.1093/nar/21.22.5025. Nucleic Acids Res. 1993. PMID: 8255756 Free PMC article.
-
The FIS protein binds and bends the origin of chromosomal DNA replication, oriC, of Escherichia coli.Nucleic Acids Res. 1991 Aug 11;19(15):4167-72. doi: 10.1093/nar/19.15.4167. Nucleic Acids Res. 1991. PMID: 1870971 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
