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. 2009:525:499-508, xiii.
doi: 10.1007/978-1-59745-554-1_26.

Scaling-up and production of therapeutic antibodies for preclinical studies

Yang Feng  1 , Dimiter S Dimitrov
Affiliations

Scaling-up and production of therapeutic antibodies for preclinical studies

Yang Feng et al. Methods Mol Biol. 2009.

Abstract

Monoclonal antibodies are increasingly used for treatment of various diseases, mostly cancer therapy and auto-immune diseases. New chimeric, humanized, or fully-human antibodies specific to novel or already validated targets are being developed in many academic labs as well as in commercial entities. A validation of antibodies in preclinical settings is an important component of the road from discovery to clinic. Preparing sufficient amount of high-quality antibodies for preclinical studies is a challenge in some cases. This article summarizes protocols used in research laboratories, and is based on our own experience in the establishment of stable clones of CHO cells to scaling-up of tissue cultures for production of antibodies in bioreactors.

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