Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Jan;37(1):411-5.
doi: 10.1007/s11033-009-9481-y. Epub 2009 Feb 28.

Preliminary study on polymorphism analysis of SpRunt-1 gene by PCR-SSCP in Strongylocentrotus intermedius and its association with growth traits

Xiuli Wang  1 , Xuemei Qiu, Xiangying Meng, Lei Yang
Affiliations

Preliminary study on polymorphism analysis of SpRunt-1 gene by PCR-SSCP in Strongylocentrotus intermedius and its association with growth traits

Xiuli Wang et al. Mol Biol Rep. 2010 Jan.

Abstract

In current study, the SpRunt-1 gene was screened for the polymorphisms using PCR-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing methods in Strongylocentrotus intermedius (S. intermedius). Three polymorphisms were found with two site mutations, G833A and C1505T mutation, and an insertion mutation (GT) between 931 nt and 932 nt. These two site mutations are synonymous mutations and the insertion mutation is frame shift mutation. One way ANOVA analysis of correlation between the single nucleotide polymorphisms (SNPs) and growth traits showed that the gonad weight of AA genotype (G at 833 nt) was significantly higher than that of BB genotype (A at 833 nt) (P = 0.029). The body weight of the CC genotype (C at 1505 nt) was significantly higher than that of DD genotype (T at 1505 nt) (P < 0.01) and the shell height of CD genotype was significantly higher than that of DD genotype (P = 0.032). These results provided the evidence that S. intermedius SpRunt-1 gene could be selected as a candidate gene for the growth traits.

PubMed Disclaimer

References

    1. BMC Med Genet. 2008 Mar 20;9:18 - PubMed
    1. Yi Chuan. 2006 Dec;28(12):1505-12 - PubMed
    1. Mech Dev. 2002 Sep;117(1-2):327-30 - PubMed
    1. Dev Biol. 1996 Feb 25;174(1):43-54 - PubMed
    1. Biochemistry (Mosc). 2003 May;68(5):582-92 - PubMed

Publication types

MeSH terms

Substances