The reactivity of EDTA, copper ion, and copper citrate with metallothioneins isolated from the digestive gland of cadmium-contaminated lobster (Homarus americanus)
- PMID: 1925524
- DOI: 10.1016/0048-9697(91)90329-d
The reactivity of EDTA, copper ion, and copper citrate with metallothioneins isolated from the digestive gland of cadmium-contaminated lobster (Homarus americanus)
Abstract
The reactivity of EDTA, Cu2+, and copper citrate with two metallothionein preparations (MT-1 and MT-2) isolated from the digestive gland of Cd-contaminated American lobster (Homarus americanus) was studied. Under pseudo-first-order conditions, metallothioneins reacted with EDTA for removal of Cd2+ in a multiphasic manner. Cadmium(+II) removal by Cu2+ was complex and non-stoichiometric, suggesting different binding sites. Rabbit liver metallothionein reacted similarly. Cadmium removal from lobster metallothionein by copper citrate was slow and triphasic in nature. EDTA removed Cu2+ from lobster metallothionein very slowly.
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