Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 May;53(5):1926-30.
doi: 10.1128/AAC.01243-08. Epub 2009 Mar 9.

Plasmodium falciparum Na+/H+ exchanger 1 transporter is involved in reduced susceptibility to quinine

Maud Henry  1 , Sébastien Briolant, Agnès Zettor, Stéphane Pelleau, Meili Baragatti, Eric Baret, Joel Mosnier, Rémy Amalvict, Thierry Fusai, Christophe Rogier, Bruno Pradines
Affiliations

Plasmodium falciparum Na+/H+ exchanger 1 transporter is involved in reduced susceptibility to quinine

Maud Henry et al. Antimicrob Agents Chemother. 2009 May.

Abstract

Polymorphisms in the Plasmodium falciparum crt (Pfcrt), Pfmdr1, and Pfmrp genes were not significantly associated with quinine (QN) 50% inhibitory concentrations (IC(50)s) in 23 strains of Plasmodium falciparum. An increased number of DNNND repeats in Pfnhe-1 microsatellite ms4760 was associated with an increased IC(50) of QN (P = 0.0007). Strains with only one DNNND repeat were more susceptible to QN (mean IC(50) of 154 nM). Strains with two DNNND repeats had intermediate susceptibility to QN (mean IC(50) of 548 nM). Strains with three DNNND repeats had reduced susceptibility to QN (mean IC(50) of 764 nM). Increased numbers of NHNDNHNNDDD repeats were associated with a decreased IC(50) of QN (P = 0.0020). Strains with profile 7 for Pfnhe-1 ms4760 (ms4760-7) were significantly associated with reduced QN susceptibility (mean IC(50) of 764 nM). The determination of DNNND and NHNDNHNNDDD repeats in Pfnhe-1 ms4760 could be a good marker of QN resistance and provide an attractive surveillance method to monitor temporal trends in P. falciparum susceptibility to QN. The validity of the markers should be further supported by analyzing more isolates.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Sequences of Pfnhe-1 microsatellite ms4760 detected among the 23 P. falciparum strains studied.
See this image and copyright information in PMC

References

    1. Abdi, A. 2007. Bonferroni and Sidak corrections for multiple comparisons, p. 1-9. In N. J. Salkind (ed.), Encyclopedia of measurement and statistics. Sage, Thousand Oaks, CA.
    1. Anderson, T. J., S. Nair, H. Qin, S. Singlam, A. Brockman, L. Paiphun, and F. Nosten. 2005. Are transporter genes other than the chloroquine resistance locus (pfcrt) and multidrug resistance gene (pfmdr) associated with antimalarial drug resistance? Antimicrob. Agents Chemother. 49:2180-2188. - PMC - PubMed
    1. Bennett, T. N., J. Patel, M. T. Ferdig, and P. D. Roepe. 2007. Plasmodium falciparum Na(+)/H(+) exchanger activity and quinine resistance. Mol. Biochem. Parasitol. 153:48-58. - PMC - PubMed
    1. Bjorkman, A., and P. A. Phillips-Howard. 1990. The epidemiology of drug-resistant malaria. Trans. R. Soc. Trop. Med. Hyg. 84:177-180. - PubMed
    1. Brasseur, P., J. Kouamouo, R. Moyou-Somo, and P. Druilhe. 1992. Multi-drug resistant falciparum malaria in Cameroon in 1987-1988. I. Stable figures of prevalence of chloroquine- and quinine-resistant isolates in the original foci. Am. J. Trop. Med. Hyg. 46:1-7. - PubMed

Publication types

MeSH terms