Responses of hatchling Xenopus tadpoles to water currents: first function of lateral line receptors without cupulae

Abstract

At later stages in larval life and also as adults, Xenopus can respond to water currents detected by their lateral-line sensory system. We have investigated when responses to water currents first appear and whether the first lateral line neuromasts operate in the same way as the adult organs. Just before and after hatching from their egg membranes we show that Xenopus embryos and tadpoles can respond to water currents by swimming into them. Local stimulation in immobilised animals where motor activity was recorded electrically suggested that the receptors detecting water currents were located between the eyes and the gills and were innervated by cranial nerves. In behaving tadpoles, responses to water currents were reduced following skin abrasion caudal to the eyes or treatment with neomycin, which is known to block hair cell function. We therefore used scanning electron microscopy to establish that rows of lateral line neuromasts with hair cells and kinocilia are present just caudal to the eyes at these stages of development. However, careful observations and manipulations of the kinocilia of neuromasts in living tadpoles failed to find any evidence that kinocilia were embedded in a jelly-like cupula. We conclude that, when they first start to function, these early neuromasts detect water movements which directly move their freely exposed hair cell kinocilia projecting out from the skin surface. Possible behavioural roles for the tadpoles responses to water are discussed.