Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Mar;10(3):193-202.
doi: 10.1631/jzus.B0820179.

Protective effects of Salvia miltiorrhizae on the hearts of rats with severe acute pancreatits or obstructive jaundice

Xi-ping Zhang  1 , Guang-hua Feng, Jie Zhang, Yang Cai, Hua Tian, Xiao-feng Zhang, Yi-feng Zhou, Zhi-wei Wang, Ke-yi Wang
Affiliations

Protective effects of Salvia miltiorrhizae on the hearts of rats with severe acute pancreatits or obstructive jaundice

Xi-ping Zhang et al. J Zhejiang Univ Sci B. 2009 Mar.

Abstract

Objective: To investigate the therapeutic effects and mechanisms of Salvia miltiorrhizae (Danshen) in the treatment of severe acute pancreatitis (SAP)- or obstructive jaundice (OJ)-induced heart injury.

Methods: A total of 288 rats were used for SAP- (n=108) and OJ-associated (n=180) experiments. The rats were randomly divided into sham-operated, model control, and Salvia miltiorrhizae-treated groups. According to the difference of time points after operation, SAP rats in each group were subdivided into 3, 6 and 12 h subgroups (n=12), whereas OJ rats were subdivided into 7, 14, 21, and 28 d subgroups (n=15). At the corresponding time points after operation, the mortality rates of the rats, the contents of endotoxin and phospholipase A2 (PLA2) in blood, and pathological changes of the hearts were investigated.

Results: The numbers of dead SAP and OJ rats in the treated groups declined as compared with those in the model control group, but not significantly (P>0.05). The contents of endotoxin (at 6 and 12 h in SAP rats and on 7, 14, 21, and 28 d in OJ rats, respectively) and PLA2 (at 6 and 12 h in SAP rats and on 28 d in OJ rats, respectively) in the treated group were significantly lower than those in the model control group (P<0.01 and P<0.001, respectively). Besides, myocardial pathological injuries were mitigated in SAP and OJ rats.

Conclusion: In this study, we found that Salvia miltiorrhizae improved myocardial pathological changes, reduced the content of PLA2 in blood, and decreased the mortality rates of SAP and OJ rats, exerting protective effects on the hearts of the rats.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Electron microscopic analyses of the model control SAP group at 12 h after operation (a) Apoptosis of myocardial cells, dissolution of mitochondrial cristae as well as swelling and vacuolation of some mitochondria; (b) Disordered arrangement of myofilaments and sarcomeres; (c) Mictochondrial swelling and vacuolation as well as disordered arrangement of myofilaments and sarcomeres
Fig. 1
Fig. 1
Electron microscopic analyses of the model control SAP group at 12 h after operation (a) Apoptosis of myocardial cells, dissolution of mitochondrial cristae as well as swelling and vacuolation of some mitochondria; (b) Disordered arrangement of myofilaments and sarcomeres; (c) Mictochondrial swelling and vacuolation as well as disordered arrangement of myofilaments and sarcomeres
Fig. 1
Fig. 1
Electron microscopic analyses of the model control SAP group at 12 h after operation (a) Apoptosis of myocardial cells, dissolution of mitochondrial cristae as well as swelling and vacuolation of some mitochondria; (b) Disordered arrangement of myofilaments and sarcomeres; (c) Mictochondrial swelling and vacuolation as well as disordered arrangement of myofilaments and sarcomeres
Fig. 2
Fig. 2
Electron microscopic analyses of the Salvia miltiorrhizae-treated SAP group at 12 h after operation (a) Ordered arrangement of myofilaments, sarcomeres and mitochondria; (b) Disordered arrangement of myocardial fibers, clear sarcomeres, abundant mitochondria, and intact structure
Fig. 2
Fig. 2
Electron microscopic analyses of the Salvia miltiorrhizae-treated SAP group at 12 h after operation (a) Ordered arrangement of myofilaments, sarcomeres and mitochondria; (b) Disordered arrangement of myocardial fibers, clear sarcomeres, abundant mitochondria, and intact structure
Fig. 3
Fig. 3
Electron microscopic analyses of the model control OJ group on 28 d after operation (a) Apoptosis of myocardial cells, dissolution of mitochondrial cristae as well as swelling and vacuolation of some mitochondria; (b) Disordered arrangement of myofilaments and sarcomeres; (c) Apoptosis of myocardial cells and disappearance of mitochondrial cristae
Fig. 3
Fig. 3
Electron microscopic analyses of the model control OJ group on 28 d after operation (a) Apoptosis of myocardial cells, dissolution of mitochondrial cristae as well as swelling and vacuolation of some mitochondria; (b) Disordered arrangement of myofilaments and sarcomeres; (c) Apoptosis of myocardial cells and disappearance of mitochondrial cristae
Fig. 3
Fig. 3
Electron microscopic analyses of the model control OJ group on 28 d after operation (a) Apoptosis of myocardial cells, dissolution of mitochondrial cristae as well as swelling and vacuolation of some mitochondria; (b) Disordered arrangement of myofilaments and sarcomeres; (c) Apoptosis of myocardial cells and disappearance of mitochondrial cristae
Fig. 4
Fig. 4
Electron microscopic analyses of the Salvia miltiorrhizae-treated OJ group on 28 d after operation (a) Mictochondrial swelling and disordered arrangement of myofilaments and sarcomeres; (b) Normal structure of myocardium
Fig. 4
Fig. 4
Electron microscopic analyses of the Salvia miltiorrhizae-treated OJ group on 28 d after operation (a) Mictochondrial swelling and disordered arrangement of myofilaments and sarcomeres; (b) Normal structure of myocardium
See this image and copyright information in PMC

References

    1. Aho HJ. Experimental pancreatitis in the rats: sodium taurocholate-induced acute hemorrhagic pancreatitis. Scand J Gastroenterol. 1980;15(4):411–416. - PubMed
    1. Assimakopoulos SF, Mavrakis AG, Grintzalis K, Papapostolou I, Zervoudakis G, Konstantinou D, Chroni E, Vagianos CE, Georgiou C. Superoxide radical formation in diverse organs of rats with experimentally induced obstructive jaundice. Redox Rep. 2008;13(4):179–184. doi: 10.1179/135100008X308902. - DOI - PubMed
    1. Celebi F, Ozturk G, Kizilkaya M. The effects of combined use of glutamine and growth hormone on the bacterial translocation associated with obstructive jaundice. Saud Med J. 2008;29(7):989–993. - PubMed
    1. de Windt LJ, Willems J, Roemen TH, Coumans WA, Reneman RS, van der Vusse GJ, van Bilsen M. Ischemic-reperfused isolated working mouse hearts: membrane damage and type IIA phospholipase A2. Am J Physiol Heart Circ Physiol. 2001;280(6):H2572–H2580. - PubMed
    1. Fuentes-Orozco C, Cervantes-Guevara G, Muciño-Hernández I, López-Ortega A, Ambriz-González G, Gutiérrez-de-la-Rosa JL, Gómez-Herrera E, Hermosillo-Sandoval JM, González-Ojeda A. L-alanyl-L-glutamine-supplemented parenteral nutrition decreases infectious morbidity rate in patients with severe acute pancreatitis. JPEN J Parenteral Enteral Nutr. 2008;32(4):403–411. doi: 10.1177/0148607108319797. - DOI - PubMed

Publication types

MeSH terms