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. 2009 May;75(10):3355-7.
doi: 10.1128/AEM.02819-08. Epub 2009 Mar 13.

Dual role of the oligopeptide permease Opp3 during growth of Staphylococcus aureus in milk

Elise Borezée-Durant  1 , Aurelia Hiron, Jean-Christophe Piard, Vincent Juillard
Affiliations

Dual role of the oligopeptide permease Opp3 during growth of Staphylococcus aureus in milk

Elise Borezée-Durant et al. Appl Environ Microbiol. 2009 May.

Abstract

Staphylococcus aureus RN6390 presents a diauxic growth in milk, due to amino acid limitation. Inactivation of the oligopeptide permease Opp3 (dedicated to the nitrogen nutrition of the strain) not only affects the growth of the strain but also results in reduced expression levels of three major extracellular proteases.

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Figures

FIG. 1.
FIG. 1.
Growth of the S. aureus RN6390 wild-type strain in milk (•) or in milk supplemented with a mixture of 18 amino acids (▴).
FIG. 2.
FIG. 2.
Growth of S. aureus RN6390 wild-type (•) and dtpT (▪), opp3 (▴), and opp3 dtpT (♦) mutant strains during growth in milk.
FIG. 3.
FIG. 3.
Effect of opp3 deletion on expression of sspA (black bars) and aur (gray bars) by S. aureus RN6390 grown in milk. Expression levels of sspA and aur in the S. aureus wild-type and opp3 mutant strains were estimated by quantitative PCR. For each strain and each time point, gene expression was normalized using gyrB as the stably expressed reference gene. Results are presented as ratios of gene expression (wild-type [wt] strain/opp3 mutant). Vertical bars indicate the standard deviations.
See this image and copyright information in PMC

References

    1. Arnaud, M., A. Chastanet, and M. Debarbouille. 2004. New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteria. Appl. Environ. Microbiol. 70:6887-6891. - PMC - PubMed
    1. Brinkman, A. B., T. J. Ettema, W. M. de Vos, and J. van der Oost. 2003. The Lrp family of transcriptional regulators. Mol. Microbiol. 48:287-294. - PubMed
    1. De Buyser, M. L., B. Dufour, M. Maire, and V. Lafarge. 2001. Implication of milk and milk products in food-borne diseases in France and in different industrialised countries. Int. J. Food Microbiol. 67:1-17. - PubMed
    1. Guedon, E., P. Serror, S. D. Ehrlich, P. Renault, and C. Delorme. 2001. Pleiotropic transcriptional repressor CodY senses the intracellular pool of branched-chain amino acids in Lactococcus lactis. Mol. Microbiol. 40:1227-1239. - PubMed
    1. Hiron, A., E. Borezee-Durant, J. C. Piard, and V. Juillard. 2007. Only one of four oligopeptide transport systems mediates nitrogen nutrition in Staphylococcus aureus. J. Bacteriol. 189:5119-5129. - PMC - PubMed

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