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. 2009 Jul;105(1):255-9.
doi: 10.1007/s00436-009-1388-y. Epub 2009 Mar 14.

Development of a real-time PCR for the differentiation of the G1 and G2/G3 genotypes of Echinococcus granulosus

Maria Paola Maurelli  1 , Laura Rinaldi, Federico Capuano, Anna Giannina Perugini, Giuseppe Cringoli
Affiliations

Development of a real-time PCR for the differentiation of the G1 and G2/G3 genotypes of Echinococcus granulosus

Maria Paola Maurelli et al. Parasitol Res. 2009 Jul.

Abstract

The present study was aimed at developing a SYBR Green-based real-time polymerase chain reaction (PCR) assay for a rapid differentiation of the genotype G1 from the cluster of genotypes G2/G3 of Echinococcus granulosus, using as marker the 12S mtDNA gene. Eleven hydatid cysts from water buffaloes and 19 from cattle were used. Fourteen samples (identified as G1 using sequencing) showed a mean melting temperature (T (m)) of 76.4 degrees C and 16 samples (identified as G2/G3 using sequencing) showed a mean T (m) of 77.0 degrees C. The detected mean difference of the T (m) of 0.6 degrees C between G1 and G2/G3 genotypes might allow a fast and simple discrimination of these genotypes. In conclusion, the real-time PCR developed in the present study provides a powerful tool for molecular studies on E. granulosus with possibilities for extension to other genotypes using different molecular targets.

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