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. 2009;23(2):139-43.
doi: 10.1002/jcla.20304.

Detection of Paracoccidioides brasiliensis gp43 gene in sputa by loop-mediated isothermal amplification method

B T Tatibana  1 , A Sano, J Uno, K Kamei, T Igarashi, Y Mikami, M Miyaji, K Nishimura, E N Itano
Affiliations

Detection of Paracoccidioides brasiliensis gp43 gene in sputa by loop-mediated isothermal amplification method

B T Tatibana et al. J Clin Lab Anal. 2009.

Abstract

The fungus Paracoccidioides brasiliensis is the pathogen of paracoccidioidomycosis (PCM), a systemic mycosis prevalent in Latin America. The loop-mediated isothermal amplification method (LAMP) was used in this study to detect the presence of P. brasiliensis in sputa samples from patients with chronic PCM, suspected PCM, and a negative control. The target P. brasiliensis gp43 gene was amplified in less than 4 hr in 11 of 18 sputa samples tested. The LAMP method had the advantage of speed and simplicity compared with the classic diagnostic methods such as the histopathological test or biological material culture and did not require sophisticated technical apparatus. It would be an important aid in cases where immediate treatment would mean patient survival, especially in immune-suppressed patients.

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References

    1. Restrepo A, McEwen JG, Castaneda E. The habitat of Paracoccidioides brasiliensis: How far from solving the riddle? Med Mycol 2001;39:233–241. - PubMed
    1. Guezelle E. Aislamiento de Paracoccidioides sp. de heces de pinguino de la Antártida, res. B2. In: Resúmenes IV Encuentro Internacional sobre Paracoccidioidomicosis. Caracas: Venezuela, 1989.
    1. Terçarioli GR, Bagagli E, Reis GM, et al. Ecological study of Paracoccidioides brasiliensis in soil: Growth ability, conidia production and molecular detection. BMC Microbiol 2007;7 :92. - PMC - PubMed
    1. Ferreira MS, Freitas LH, Lacaz CS, et al. Isolation and characterization of a Paracoccidioides brasiliensis strain from a dog food probably contaminated with soil in Uberlandia, Brazil. J Med Vet Mycol 1990;28:253–256. - PubMed
    1. Grosse E, Tamsitt J. Paracoccidioides brasiliensis recovered from the intestinal tract of three bats (Artibeus lituratus) in Colombia S.A. Sabouraudia 1965;4:124–125. - PubMed