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. 1977 Apr;22(1):113-7.
doi: 10.1128/JVI.22.1.113-117.1977.

Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel

B Norrild, B F Vestergaard

Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel

B Norrild et al. J Virol. 1977 Apr.

Abstract

Crossed immunoelectrophoresis was used to characterize herpes simplex virus type 1 (HSV-1) antigens produced by infected HEp-2 cells. We report on a method for analyzing the polypeptide content in individual antigen-antibody precipitates eluted from the second-dimensional agarose gel. Four glycoprotein antigens of HSV-1, Ag-8, Ag-11, Ag-6, and Ag-3, were isolated and analyzed for polypeptide content. The molecular weights of the polypeptides are presented.

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References

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    1. IARC Sci Publ. 1975;(11 Pt 1):3-38 - PubMed
    1. J Gen Virol. 1975 Nov;29(2):167-78 - PubMed
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