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. 2009 Mar 16:8:46.
doi: 10.1186/1475-2875-8-46.

Anopheles pseudowillmori is the predominant malaria vector in Motuo County, Tibet Autonomous Region

Song Wu  1 , Jia-Yun Pan, Xue-Zhong Wang, Shui-Sen Zhou, Guo-Qing Zhang, Qian Liu, Lin-Hua Tang
Affiliations

Anopheles pseudowillmori is the predominant malaria vector in Motuo County, Tibet Autonomous Region

Song Wu et al. Malar J. .

Abstract

Background: Malaria is endemic in Linzhi Prefecture in the Tibet Autonomous Region (TAR), but the vector for malaria transmission had never been identified.

Methods: Adult Anopheles spp. were collected in Motuo County, Linzhi Prefecture on the Sino-Indian border in July and August, 2007. Multiplex PCR was adopted for species identification, and a nested PCR approach was used to detect sporozoites in the salivary glands of the mosquitoes.

Results: 3,675 mosquitoes of the Anopheles maculatus group were collected and processed for species identification. Among them, 3,602 (98.0%) were Anopheles pseudowillmori and 73 (2.0%) were Anopheles willmori. The Plasmodium vivax SSUrDNA fragment was amplified in two of 360 pooled An. pseudowillmori samples.

Conclusion: The local An. maculatus group comprises the species An. pseudowillmori and An. willmori. Anopheles pseudowillmori is considered the sole malaria vector in Motuo County in Linzhi Prefecture.

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Figures

Figure 1
Figure 1
The map of relative study sites.
Figure 2
Figure 2
Anopheles maculatus s.l. species identification by PCR. Ethidium bromide-stained PCR products from five species belonging to An. maculatus s.l. as well as positive samples collected in Motuo County, TAR after electrophoresis on agarose gel. Lane M: 100 bp ladder; lane 1: An. pseudowillmori; lane 2: An. maculatus s.s.; lane 3: An. willmori; lane 4: An. dravidicus; lane 5: An. sawadwongporni; lanes 6–8: Anopheles spp. collected in Motuo County, TAR; lane 9: negative control.
Figure 3
Figure 3
Sporozoite detection by PCR. Ethidium bromide-stained PCR products from the nested PCR approach for sporozoite detection. Lane M: 100 bp ladder; lane 1: positive control; lanes 2–3: pooled samples from Motuo County, TAR; lane 4: negative control.
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