doi: 10.1139/w08-113.
Detection of Bacillus sphaericus mosquitocidal toxin genes by multiplex colony PCR
Affiliations
- PMID: 19295654
- DOI: 10.1139/w08-113
Item in Clipboard
Detection of Bacillus sphaericus mosquitocidal toxin genes by multiplex colony PCR
Can J Microbiol.
2009 Feb.
Abstract
A multiplex colony PCR assay was developed for the detection of 5 genes encoding Bacillus sphaericus mosquito larvicidal toxins, namely binA, binB, mtx1, mtx2, and mtx3. Primers designed for these 5 genes yielded specific PCR amplicons of the expected size from type cultures of B. sphaericus. This method of detecting multiple toxin genes by colony PCR in a single tube reaction is a simple, rapid, and economical technique for identification of highly toxic environmental B. sphaericus isolates.
Similar articles
-
Molecular characterization of mosquitocidal Bacillus sphaericus isolated from Tamil Nadu, India.Acta Trop. 2013 Sep;127(3):158-64. doi: 10.1016/j.actatropica.2013.04.013. Epub 2013 May 3. Acta Trop. 2013. PMID: 23648218
-
Bacillus sphaericus Mtx1 and Mtx2 toxins co-expressed in Escherichia coli are synergistic against Aedes aegypti larvae.Biotechnol Lett. 2009 Apr;31(4):551-5. doi: 10.1007/s10529-008-9896-x. Epub 2008 Dec 11. Biotechnol Lett. 2009. PMID: 19082531
-
Location of 43 kD mosquito-larvicidal toxin gene of highly toxic Bacillus sphaericus 10.Sci China B. 1991 May;34(5):593-8. Sci China B. 1991. PMID: 2059322
-
New gene from nine Bacillus sphaericus strains encoding highly conserved 35.8-kilodalton mosquitocidal toxins.Appl Environ Microbiol. 1996 Jun;62(6):2174-6. doi: 10.1128/aem.62.6.2174-2176.1996. Appl Environ Microbiol. 1996. PMID: 8787415 Free PMC article.
-
Hexaplex PCR for rapid detection of virulence factors.Expert Rev Mol Diagn. 2003 Nov;3(6):781-4. doi: 10.1586/14737159.3.6.781. Expert Rev Mol Diagn. 2003. PMID: 14628905 Review.
Cited by
-
Allelic diversity and population structure of Bacillus sphaericus as revealed by multilocus sequence typing.Appl Environ Microbiol. 2011 Aug;77(15):5553-6. doi: 10.1128/AEM.00207-11. Epub 2011 Jun 17. Appl Environ Microbiol. 2011. PMID: 21685170 Free PMC article.
-
Identification of a Novel Brevibacillus laterosporus Strain With Insecticidal Activity Against Aedes albopictus Larvae.Front Microbiol. 2021 Feb 17;12:624014. doi: 10.3389/fmicb.2021.624014. eCollection 2021. Front Microbiol. 2021. PMID: 33679643 Free PMC article.
-
Identification of mosquito larvicidal bacterial strains isolated from north Sinai in Egypt.AMB Express. 2012 Jan 26;2:9. doi: 10.1186/2191-0855-2-9. AMB Express. 2012. PMID: 22280528 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
