Arsenic accumulation and speciation in maize as affected by inoculation with arbuscular mycorrhizal fungus Glomus mosseae

Abstract

Effects of inoculation with arbuscular mycorrhizal (AM) fungus (Glomus mosseae) on arsenic (As) accumulation and speciation in maize were investigated by using As spiked soil at the application levels of 0, 25, 50, and 100 mg kg(-1). Inorganic As was the major species in plants, and mycorrhizal inoculation generally decreased concentrations of arsenite [As(III)] in maize roots and concentrations of As(III) and arsenate [As(V)] in the shoots. Dimethylarsenic acid (DMA) concentrations (detected in every plant sample) were higher in maize shoots for mycorrhizal than for nonmycorrhizal treatment, but no significant differences were observed for roots. Monomethylarsenic acid (MMA) was only detected in roots with mycorrhizal colonization. The uptake of As(V) was much lower by excised mycorrhizal than nonmycorrhizal roots, and the differences for the uptake of As(III) were negligible. Arsenate reductase (AR) activity was detected in maize roots, and it was reduced with mycorrhizal inoculation. Activities of peroxidase (POD) and superoxide dismutase (SOD) were detected in both maize shoots and roots, and they were suppressed by mycorrhizal inoculation. AM inoculation inhibited the uptake of As(V) and its reduction to As(III), reducing oxidation stress and thereby alleviating As toxicity to the host plant.