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Review
. 2009 Apr 15;877(11-12):1019-39.
doi: 10.1016/j.jchromb.2009.02.050. Epub 2009 Feb 27.

Highly efficient peptide separations in proteomics. Part 2: bi- and multidimensional liquid-based separation techniques

Koen Sandra  1 , Mahan Moshir, Filip D'hondt, Robin Tuytten, Katleen Verleysen, Koen Kas, Isabelle François, Pat Sandra
Affiliations
Review

Highly efficient peptide separations in proteomics. Part 2: bi- and multidimensional liquid-based separation techniques

Koen Sandra et al. J Chromatogr B Analyt Technol Biomed Life Sci. .

Abstract

Multidimensional liquid-based separation techniques are described for maximizing the resolution of the enormous number of peptides generated upon tryptic digestion of proteomes, and hence, reduce the spatial and temporal complexity of the sample to a level that allows successful mass spectrometric analysis. This review complements the previous contribution on unidimensional high performance liquid chromatography (HPLC). Both chromatography and electrophoresis will be discussed albeit with reversed-phase HPLC (RPLC) as the final separation dimension prior to MS analysis.

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