Ethanol-induced social facilitation in adolescent rats: role of endogenous activity at mu opioid receptors

Abstract

Background: Ethanol consumption is considerably elevated during adolescence. Attractiveness of alcohol for humans during the adolescent developmental period is based, in part, on its ability to induce social facilitation--a facilitation of social interactions not only evident in human adolescents but also in adolescent rats. Endogenous opioid systems are among the multiple neural systems implicated in the behavioral and reinforcing effects of ethanol and may play a substantial role in modulating stimulatory effects of low doses of ethanol on social behavior during adolescence. This possibility was explored in the present study through the use of an animal model of peer-directed social behavior.

Methods: Sprague-Dawley rats were challenged early in adolescence with saline or ethanol intraperitoneally (i.p.), placed into an individual holding cage for 30 minutes, and then tested in a familiar situation with a nonmanipulated partner of the same age and sex. In Experiment 1, each test subject was injected subcutaneously with one of the three doses of a nonselective opioid antagonist naloxone (0, 0.05, and 0.1 mg/kg), 5 minutes prior to the social interaction test and 25 minutes following challenge with saline or ethanol (0.5 g/kg), whereas in Experiment 2 animals were challenged with one of the six doses of ethanol (0, 0.25, 0.5, 0.75, 1.0, and 1.25 g/kg) prior to injection of either saline or naloxone (0.05 mg/kg). In Experiment 3, animals were pretreated i.p. with the selective mu-opioid antagonist CTOP (0, 0.01, 0.025, 0.05, and 0.1 mg/kg) 30 minutes prior to challenge with saline or ethanol (0.5 g/kg).

Results: Low doses of ethanol (0.5 and 0.75 g/kg) produced social facilitation, as indexed by significant increases in play fighting and social investigation. Both doses of naloxone and the three highest doses of CTOP blocked the stimulatory effects of ethanol on play fighting but not on social investigation. These effects were not associated with alterations in ethanol pharmacokinetic properties or with shifts in the biphasic ethanol dose-response curve.

Conclusions: Ethanol-induced facilitation of social play behavior seen in adolescent animals is mediated in part through ethanol-induced release of endogenous ligands for the mu-opioid receptor or an ethanol-associated enhancement of sensitivity of these receptors for their endogenous ligands.

Fig. 1

Social investigation (left), play fighting (middle), and overall locomotor activity (right) for adolescent rats challenged either with saline or 0.5 g/kg ethanol and injected with one of the three doses of naloxone in Experiment 1. Significant changes induced by ethanol challenge are marked by asterisks, p < 0.05.

Fig. 2

Social investigation (left) and play fighting (right) for adolescent rats challenged with one of the five doses of ethanol or saline and injected either with saline or naloxone pretest in Experiment 2. Significant changes induced by ethanol challenge are marked by asterisks, p < 0.05.

Fig. 3

Locomotor activity (left) for adolescent rats challenged with one of the five doses of ethanol or saline and injected either with saline or naloxone pretest in Experiment 2. Significant changes induced by ethanol challenge are marked by asterisks, p < 0.05. Blood ethanol concentrations (right) are presented for animals challenged with ethanol.

Fig. 4

Social investigation (left) and play fighting (right) for adolescent rats challenged with either ethanol or saline and pretreated with one of the five doses of CTOP in Experiment 3. Significant changes induced by ethanol challenge are marked by asterisks, p < 0.05.

Fig. 5

Locomotor activity (left) for adolescent rats challenged with either ethanol or saline and pretreated with one of the five doses of CTOP in Experiment 3. Blood ethanol concentrations (right) are presented for animals challenged with ethanol.