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. 2009 Mar 4;583(5):909-14.
doi: 10.1016/j.febslet.2009.02.003. Epub 2009 Feb 10.

The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction

Joachim Zettler  1 , Vivien Schütz, Henning D Mootz
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Free article

The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction

Joachim Zettler et al. FEBS Lett. .
Free article

Abstract

We have studied the naturally split alpha subunit of the DNA polymerase III (DnaE) intein from Nostoc punctiforme PCC73102 (Npu) using purified proteins and determined an apparent first-order rate constant of (1.1+/-0.2)x10(-2) s(-1) at 37 degrees C. This represents the highest rate reported for the protein trans-splicing reaction so far (t(1/2) of approximately 60s). Furthermore, the reaction was very robust and high-yielding with respect to different extein sequences, temperatures from 6 to 37 degrees C, and the presence of up to 6 M urea. Given these outstanding properties, the Npu DnaE intein appears to be the intein of choice for many applications in protein and cellular chemistry.

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