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. 2009 May;75(10):3352-4.
doi: 10.1128/AEM.02407-08. Epub 2009 Mar 20.

Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system

B C Ferrari  1 , M R Gillings
Affiliations

Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system

B C Ferrari et al. Appl Environ Microbiol. 2009 May.

Abstract

Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.

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Figures

FIG. 1.
FIG. 1.
Fluorescence viability staining of microcolonies following microcultivation using the SSMS system. Live mCFUs (green) were easily differentiated from dead mCFUs (red) at a magnification of ×400. Differentiation of both live and dead cells enables specific morphotypes of mCFUs to be targeted for micromanipulation in growth membranes for subsequent cultivation in oligophilic media.
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