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. 2009 Jun;198(2):275-81.
doi: 10.1016/j.jmr.2009.02.012. Epub 2009 Mar 4.

H-start for exclusively heteronuclear NMR spectroscopy: the case of intrinsically disordered proteins

Wolfgang Bermel  1 , Ivano Bertini, Veronika Csizmok, Isabella C Felli, Roberta Pierattelli, Peter Tompa
Affiliations

H-start for exclusively heteronuclear NMR spectroscopy: the case of intrinsically disordered proteins

Wolfgang Bermel et al. J Magn Reson. 2009 Jun.

Abstract

Here, we present a series of exclusively heteronuclear multidimensional NMR experiments, based on 13C direct detection, which exploit the (1)H polarization as a starting source to increase the signal-to-noise ratio. This contributes to make this spectroscopy more useful and usable. Examples are reported for a suitable system such as securin, an intrinsically disordered protein of 22 kDa.

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