Sexing of forensic samples using PCR
- PMID: 1931736
- DOI: 10.1007/BF01369808
Sexing of forensic samples using PCR
Abstract
A rapid protocol has been established for sexing forensic samples by the Polymerase Chain Reaction method. Three sets of primer were used, two specific for Y chromosome repetitive sequences and one specific for X chromosome repetitive sequences. Detailed procedures of experiments, the controls and the applications to testing bloodstains and a vaginal swab are presented. The sensitivity of the test and problems due to contamination are discussed.
Similar articles
-
Sex determination of forensic samples: co-amplification and simultaneous detection of a Y-specific and an X-specific DNA sequence.Int J Legal Med. 1993;105(4):213-6. doi: 10.1007/BF01642796. Int J Legal Med. 1993. PMID: 8431401
-
Sex determination of forensic samples by simultaneous PCR amplification of alpha-satellite DNA from both the X and Y chromosomes.J Forensic Sci. 1995 Mar;40(2):239-41. J Forensic Sci. 1995. PMID: 7602285
-
Sex determination from blood and bloodstains by polymerase chain reaction (PCR).Nihon Hoigaku Zasshi. 1994 Feb;48(1):7-18. Nihon Hoigaku Zasshi. 1994. PMID: 8176859
-
[Interpretation of DNA profiles for mixed stains].Fa Yi Xue Za Zhi. 2002 Aug;18(3):185-8. Fa Yi Xue Za Zhi. 2002. PMID: 12608297 Review. Chinese.
-
[Application of DNA polymorphism to forensic medicine].Rinsho Byori. 1994 Jun;42(6):636-42. Rinsho Byori. 1994. PMID: 7914244 Review. Japanese.
Cited by
-
Sex determination of forensic samples: co-amplification and simultaneous detection of a Y-specific and an X-specific DNA sequence.Int J Legal Med. 1993;105(4):213-6. doi: 10.1007/BF01642796. Int J Legal Med. 1993. PMID: 8431401
-
Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin.Int J Legal Med. 1994;106(4):190-3. doi: 10.1007/BF01371335. Int J Legal Med. 1994. PMID: 8038111