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. 2009 Mar:Chapter 16:Unit 16.1.
doi: 10.1002/0471142700.nc1601s36.

Overview of gene silencing by RNA interference

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Overview of gene silencing by RNA interference

Daniel H Kim et al. Curr Protoc Nucleic Acid Chem. 2009 Mar.

Abstract

The potential of harnessing RNA interference (RNAi) for sequence-specific gene silencing has generated much excitement and progress in the field. Recent advances in RNAi technology suggest that RNAi-based approaches may soon become an effective therapeutic strategy against a myriad of diseases. This overview provides a brief description of important considerations when designing an RNAi-based method for gene silencing and therapeutic development: (a) mechanistic aspects of RNAi-mediated gene silencing in mammalian cells; (b) structural requirements for potent siRNA duplexes; (c) off-target effects and interferon responses; and (d) effective delivery of RNAi-inducing agents. Promising therapeutic applications of RNAi that are currently in the developmental pipeline are also described.

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