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Comparative Study
. 1991 Oct 8;1090(2):277-80.
doi: 10.1016/0167-4781(91)90119-7.

Isolation and characterisation of a bovine cDNA encoding eukaryotic initiation factor 2 alpha

S R Green  1 , J Fullekrug, K Sauer, M F Tuite
Affiliations
Comparative Study

Isolation and characterisation of a bovine cDNA encoding eukaryotic initiation factor 2 alpha

S R Green et al. Biochim Biophys Acta. .

Abstract

Two cDNA clones have been isolated, from a bovine lymphosarcoma library, that encode the alpha-subunit of eukaryotic initiation factor 2 (eIF-2 alpha). The predicted 315 amino acid sequence showed more than 99% amino acid identity with rat and human eIF-2 alpha. Galactose-regulated expression of a full length bovine eIF-2 alpha cDNA in yeast resulted in the synthesis of a polypeptide of the predicted molecular mass (36 kDa). Furthermore, the expressed polypeptide cross-reacted with an antibody raised against rabbit eIF-2 alpha confirming the identity of the cDNA.

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