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. 2009 Mar 24;14(3):1279-87.
doi: 10.3390/molecules14031279.

Thermus thermophilus strains active in purine nucleoside synthesis

Marcos Almendros  1 , José-Vicente Sinisterra Gago, José Berenguer Carlos
Affiliations

Thermus thermophilus strains active in purine nucleoside synthesis

Marcos Almendros et al. Molecules. .

Abstract

Several strains of Thermus thermophilus were tested in order to detect purine nucleoside synthase activity using pyrimidine nucleosides as the sugar-donor and adenine or hypoxanthine as bases. High productivity values (t =1 hr) were obtained while completely avoiding adenosine-deaminase degradation of the products. N-2-deoxy-ribosyltransferase activity is described for the first time in hyperthermophilic bacteria.

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Figures

Scheme 1
Scheme 1
Reaction pathways of the adenine nucleoside synthesis and degradation of adenine and adenine nucleosides by ADA.
Scheme 2
Scheme 2
One-pot enzymatic synthesis of adenine nucleosides using uridine or 2’-deoxyuridine as donors of sugar moiety.
Scheme 3
Scheme 3
Reaction mechanism of the N-2-deoxyribosyltranferase catalysis.
Figure 1
Figure 1
Influence of pH in the productivity of deoxyadenosine by T. thermophilus HB27. Standard reaction conditions: 65ºC, 5mM deoxyuridine, 5mM adenine, 30mM sodium phosphate buffer (pH=7). Total reaction volume 4 mL.
Figure 2
Figure 2
Influence of deoxyuridine/adenine ratio. Standard reaction conditions: 65ºC, 5mM deoxyuridine, 5mM adenine, 30mM sodium phosphate buffer (pH=7). Total reaction volume 4 mL.
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