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. 2009 May;5(5):341-3.
doi: 10.1038/nchembio.157. Epub 2009 Mar 29.

Ligand-directed tosyl chemistry for protein labeling in vivo

Shinya Tsukiji  1 , Masayoshi Miyagawa, Yousuke Takaoka, Tomonori Tamura, Itaru Hamachi
Affiliations

Ligand-directed tosyl chemistry for protein labeling in vivo

Shinya Tsukiji et al. Nat Chem Biol. 2009 May.

Abstract

Here we describe a method for the site-selective attachment of synthetic molecules into specific 'endogenous' proteins in vivo using ligand-directed tosyl (LDT) chemistry. This approach was applied not only for chemically labeling proteins in living cells, tissues and mice but also for constructing a biosensor directly inside cells without genetic engineering. These data establish LDT chemistry as a new tool for the study and manipulation of biological systems.

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References

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