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. 2009 Mar 30;28(1):43.
doi: 10.1186/1756-9966-28-43.

Overexpression of transketolase-like gene 1 is associated with cell proliferation in uterine cervix cancer

Hui Chen  1 , Jian-Xin Yue, Shou-Hua Yang, Hui Ding, Rong-Wei Zhao, Song Zhang
Affiliations

Overexpression of transketolase-like gene 1 is associated with cell proliferation in uterine cervix cancer

Hui Chen et al. J Exp Clin Cancer Res. .

Abstract

Background: Tumor cells need large energy and nucleic acids to proliferate and grow. For most of their energy needs, cancer cells depend more on glycolysis. For most of their nucleic acids needs, cancer cells depend more on the nonoxidative pathway of the pentose phosphate pathway. Transketolase(TKT) is a crucial enzyme in the nonoxidative pathway of the PPP.

Methods: The real-time quantity PCR was used to determine the expression of transketolase gene family in uterine cervix cancer. Transketolase activity of cell was determined by using enzyme-linked method. Cell proliferation was detected by using MTT.

Results: The TKTL1 mRNA was specifically over-expressed in uterine cervix cancer cells(HeLa cell line) compare with normal human endocervical epithelial cells(End1/E6E7 cell line)(P < 0.05), whereas the expression of TKT and transketolase-like gene 2(TKTL2) have no significant differences between the two cell lines(P > 0.05). Moreover, we found that total transketolase activity was significantly reduced, and cell proliferation was remarkably inhibited after anti-TKTL1 siRNA treatment in HeLa cells. The total transketolase activity and cell proliferation have no significant differences after anti-TKTL1 siRNA treatment in End1/E6E7 cells.

Conclusion: These results indicate that TKTL1 plays an important role in total transketolase activity and cells proliferation in uterine cervix cancer.

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Figures

Figure 1
Figure 1
Expression of transketolase gene family was analyzed by using gel electrophoresis in the End1/E6E7 cells and HeLa cells. In the End1/E6E7 cells (A), the expression of TKT was significantly higher than the expression of TKTL1 and TKTL2. In the HeLa cells (B), the expression of TKTL1 was significantly higher than the expression of TKT and TKTL2. No expression of TKTL1 was found after transfected with siRNA in the End1/E6E7 cells and HeLa cells. β-actin: 520 bp, TKT: 176 bp, TKTL1: 150 bp, TKTL2: 146 bp.
Figure 2
Figure 2
The effect of anti-TKTL1 siRNA on transketolase activity in the HeLa cells and End1/E6E7 cells. 1: the cells without transfection, 2: the cells transfected control plasmid, 3: the cells transfected siRNA. The total transketolase activity was significantly increased in the HeLa cells without transfection compared to that in the End1/E6E7 cells without transfection. The total transketolase activity was significantly decreased in the HeLa cells transfected siRNA. There were no significant difference existed in total transketolase activity after transfected siRNA in the End1/E6E7 cells.
Figure 3
Figure 3
The effect of anti-TKTL1 siRNA on proliferation of End1/E6E7 cells and HeLa cells. In the End1/E6E7 cells (A), There was no significant difference of cell proliferation among the cells without transfection, transfected with control plasmid and transfected with siRNA. In the HeLa cells (B), cell proliferation was significantly inhibited after transfected siRNA TKTL1 construct.
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