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. 2009 Apr;15(4):610-1.
doi: 10.3201/eid1504.071271.

Spotted fever group Rickettsia sp. closely related to Rickettsia japonica, Thailand

Spotted fever group Rickettsia sp. closely related to Rickettsia japonica, Thailand

Nobuhiro Takada et al. Emerg Infect Dis. 2009 Apr.
No abstract available

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Figures

Figure
Figure
Phylogenetic analysis based on ompA gene (A) and rickettsial genus–specific 17-kDa antigen gene (B). Sequences were aligned by using the ClustalW software package (http://clustalw.ddbj.nig.ac.jp/top-j.html), and neighbor-joining phylogenetic tree construction and bootstrap analysis were conducted according to the Kimura 2-parameter method (www.ddbj.nig.ac.jp). Pairwise alignments were performed with an open-gap penalty of 10, a gap extension penalty of 0.5, and a gap distance of 8. Multiple alignments were also performed with the same values, and the phylogenetic branches were supported by bootstrap analysis with 1,000 replications (>800 were indicated). Rickettsia felis (CP000053) and R. canadensis (CP000409) were used as outgroups for ompA and 17-kDa antigen gene, respectively. The phylogenetic tree was constructed by using TreeView software version 1.5 (http://taxonomy.zoology.gla.ac.uk/rod/treeview.html). Scale bars indicate nucleotide substitutions (%) per site.

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References

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