doi: 10.1016/j.ab.2009.03.036.
Epub 2009 Mar 28.
Circular dichroism spectroscopy has intrinsic limitations for protein secondary structure analysis
Affiliations
- PMID: 19332020
- PMCID: PMC2696129
- DOI: 10.1016/j.ab.2009.03.036
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Circular dichroism spectroscopy has intrinsic limitations for protein secondary structure analysis
Anal Biochem.
.
Abstract
Secondary structure content (SSC) cannot be calculated accurately from circular dichroism (CD) spectra for the majority of proteins whose three-dimensional structures have been solved. "Reliable" SSC that is significantly different from random SSC can be calculated from CD spectra only for all-alpha proteins and all-beta proteins with canonical beta-strand geometry.
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