Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Jun 15;389(2):174-6.
doi: 10.1016/j.ab.2009.03.036. Epub 2009 Mar 28.

Circular dichroism spectroscopy has intrinsic limitations for protein secondary structure analysis

Sergei Khrapunov  1
Affiliations

Circular dichroism spectroscopy has intrinsic limitations for protein secondary structure analysis

Sergei Khrapunov. Anal Biochem. .

Abstract

Secondary structure content (SSC) cannot be calculated accurately from circular dichroism (CD) spectra for the majority of proteins whose three-dimensional structures have been solved. "Reliable" SSC that is significantly different from random SSC can be calculated from CD spectra only for all-alpha proteins and all-beta proteins with canonical beta-strand geometry.

PubMed Disclaimer

References

    1. Greenfield N. Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions. Nature Protocols. 2006;1:2876–2880. - PMC - PubMed
    1. Greenfield N. Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism. Nature Protocols. 2006;1:2733–2741. - PMC - PubMed
    1. Greenfield N. Using circular dichroism spectra to estimate protein secondary structure. Nature Protocols. 2006;1:2876–2890. - PMC - PubMed
    1. Sreerama N, Woody R. Estimation of protein secondary structure from circular dichroism spectra: comparison of CONTIN, SELCON, and CDSSTR methods with an expanded reference set. Analitical Biochemistry. 2000;287:252–260. - PubMed
    1. Whitmore L, Wallace B. DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data. Nucleic Acids Research. 2004;32:W668–W673. - PMC - PubMed

Publication types

MeSH terms

LinkOut - more resources