Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Mar-Apr;57(2):141-6.
doi: 10.1007/s00005-009-0015-z. Epub 2009 Mar 31.

Prophylaxis with alpha-lipoic acid against lipopolysaccharide-induced brain injury in rats

Anna Goraca  1 , Katarzyna Asłanowicz-Antkowiak
Affiliations

Prophylaxis with alpha-lipoic acid against lipopolysaccharide-induced brain injury in rats

Anna Goraca et al. Arch Immunol Ther Exp (Warsz). 2009 Mar-Apr.

Abstract

Introduction: Lipopolysaccharide (LPS) stimulates the synthesis and release of reactive oxygen species that play an important role in the pathogenesis of tissue injuries. In this study the effect of early administration of the antioxidant alpha-lipoic acid (alpha-LA) on brain lipid peroxidation, brain hydrogen peroxide (H(2)O(2)) concentration, and brain total sulfhydryl group (-SH group) content was evaluated in rats with endotoxic shock induced by administration of LPS (Escherichia coli 026:B6, 30 mg/kg i.v.)

Materials and methods: Rats were treated intravenously with normal saline or alpha-LA (60 mg/kg) 30 min after LPS injection. After 5 h of observation, the animals were killed and their brains were isolated for the measurements.

Results: Injection of LPS alone resulted in the development of shock and oxidative stress, the latter indicated by a significant increase in brain concentrations of thiobarbituric acid-reacting substances (TBARS) and H(2)O(2) and a decrease in total brain -SH group content. Administration of alpha-LA after the LPS challenge resulted in an increase in total -SH group content and a decrease in TBARS and H(2)O(2) concentration in the brain tissue compared with the LPS group.

Conclusion: The results indicate that alpha-LA treatment effectively protected the brain tissue against endotoxin-induced oxidative stress. Administration of LA could be a useful adjunct to clinical application in the management of septic shock.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1080/00207450601050287', 'is_inner': False, 'url': 'https://doi.org/10.1080/00207450601050287'}, {'type': 'PubMed', 'value': '17987471', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/17987471/'}]}
    2. Akpinar D, Yargicoglu P, Derin N et al (2007) The effect of lipoic acid on lipid peroxidation and visual evoked potentials (VEPs) in rats exposed to chronic restraint stress. Int J Neurosci 117: 1691-1706 - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1021/bi7017464', 'is_inner': False, 'url': 'https://doi.org/10.1021/bi7017464'}, {'type': 'PubMed', 'value': '18081316', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/18081316/'}]}
    2. Applegate MA, Humphries KM, Szweda LI (2008) Reversible inhibition of alpha-ketoglutarate dehydrogenase by hydrogen peroxide: glutathionylation and protection of lipoic acid.. Biochemistry 47: 473-478 - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1074/jbc.M407328200', 'is_inner': False, 'url': 'https://doi.org/10.1074/jbc.m407328200'}, {'type': 'PubMed', 'value': '15383538', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/15383538/'}]}
    2. Arai H, Furuya T, Yasuda T et al (2004) Neurotoxic effects of lipopolysaccharide on nigral dopaminergic neurons are mediated by microglial activation, interleukin 1-beta, and expression of caspase-11 in mice. J Biol Chem 279: 51647-51653 - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1016/0891-5849(94)00244-E', 'is_inner': False, 'url': 'https://doi.org/10.1016/0891-5849(94)00244-e'}, {'type': 'PubMed', 'value': '7635361', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/7635361/'}]}
    2. Benzi G, Moretti A (1995) Age- and peroxidative stress-related modifications of the cerebral enzymatic activities linked to mitochondria and the glutathione system. Free Radic Biol Med 19: 77-101 - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PubMed', 'value': '16227639', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/16227639/'}]}
    2. Bilska A, Włodek L (2005) Lipoic acid — the drug of the future?. Pharmacol Rep 57: 570-577 - PubMed

Publication types