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. 2008;3(4):461-9.
doi: 10.2147/ijn.s3607.

Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications

Varvara Karagkiozaki  1 , Stergios Logothetidis, Sylvie Lousinian, George Giannoglou
Affiliations

Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications

Varvara Karagkiozaki et al. Int J Nanomedicine. 2008.

Abstract

Electric surface properties of biomaterials, playing key role to various biointerfacial interactions, were related to hemocompatibility and biosensing phenomena. In this study, the examination of surface electric properties of amorphous hydrogenated carbon thin films (a-C:H) was carried out by means of electrostatic force microscope (EFM) and observation of differences in spatial charge distribution on the surface of the examined films during platelets adhesion was made. The thrombogenic potential of a-C:H thin films developed by magnetron sputtering with approximately 42% sp(3) content and hydrogen partial pressure during deposition was evaluated, by in situ observation with atomic force microscope (AFM) of platelets' activation and their subsequent adhesion. Platelet-rich plasma drawn from healthy donors was used and semi-contact mode of AFM was applied. Platelets behavior and their correlation with the electric surface properties of the examined a-C:H films by EFM was made for hemocompatibility enhancement and sensing platelets that are less electrical negatively charged and with higher tendency to aggregate and form thrombus. The results are discussed in view of the effect of different deposition conditions of hydrogenated carbon films on their structural and morphological characteristics, surface roughness and electrical properties attributing to different hemocompatibility and sensing aspects.

Keywords: amorphous hydrogenated carbon; atomic force microscopy; electric force microscopy; medical applications; platelets; thrombogenicity.

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Figures

Figure 1
Figure 1
Atomic force microscope topography image (10 μm × 10 μm) of (a) a-C: H (Biased) as deposited, (b) a-C:H (Floating) as deposited.
Figure 2
Figure 2
Atomic force microscope topography image of (a) platelets on a-C:H (Floating) after 1 hour incubation time (scan size 10 μm × 10 μm). Arrows indicate the platelets’ clusters. (b) Platelets on a-C:H (Floating) after 2 hours incubation time, with a size of 20 μm × 20 μm. Arrows indicate the formation of platelets’ cluster like an island.
Figure 3
Figure 3
Comparative diagram of mean Peak-to Peak parameter of examined platelets versus incubation time for the two different types of a-C:H thin films.
Figure 4
Figure 4
Comparative diagram of mean RMS roughness of examined platelets versus incubation time for the two different types of a-C:H thin films.
Figure 5
Figure 5
Atomic force microscope topography image of (a) platelets on a-C:H (Biased) after 1 hour incubation time (scan size 10 μm × 10 μm) (b) Platelets on a-C:H (Biased) after 2 hours incubation time, with a size of 20 μm × 20 μm.
Figure 6
Figure 6
Electric force microscope image: platelets on a-C:H (Floating) after 1 hour incubation time (scan size 10 μm × 10 μm) (a) MAG*COS. The blue arrows indicate the platelets’ clusters whereas the green square denotes an area free of platelets (b) Phase.
Figure 7
Figure 7
(a) Atomic force microscope topography image of platelets on a-C:H (Floating) after 1 hour incubation time (scan size 10 μm × 10 μm). Black circle indicates the platelets’ cluster. The two lines indicate the upper and lower boundaries of this platelets cluster where the x-cross sections were made, (b) X – section at 7.200 nm of Figure 7a. The blue arrow shows the height of the small red circle of the upper boundaries of the examined platelets’ aggregation, (c) X – section at 4.500 nm of Figure 7a. The red arrow is indicative of the height of the small red circle of the lower boundaries of this platelets’ cluster.
Figure 8
Figure 8
(a) Atomic force microscope topography image of platelets on a-C:H (Biased) after 1 hour incubation time (scan size 10 μm × 10 μm). Black circles are indicative of platelets. (b) Electric force microscope (MAG*COS) image of platelets on a-C:H (Floating) after 1 hour of incubation. The square shows the areas without platelets whereas the black circles indicate platelets.
Figure 9
Figure 9
Electric force microscope images of a-C:H (Biased) as deposited (scan size 10μm × 10 μm) (a) MAG*COS (b) Phase.
Figure 10
Figure 10
Electric force microscope images of a-C:H (Floating) as deposited (scan size 10 μm × 10 μm) (a) MAG*COS (b) Phase.
See this image and copyright information in PMC

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