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. 2009 Apr 7;15(13):1620-4.
doi: 10.3748/wjg.15.1620.

Altered spontaneous contractions of the ileum by anesthetic agents in rats exposed to peritonitis

Cengiz Aydin  1 , Ihsan Bagcivan, Sinan Gursoy, Ahmet Altun, Omer Topcu, Ayhan Koyuncu
Affiliations

Altered spontaneous contractions of the ileum by anesthetic agents in rats exposed to peritonitis

Cengiz Aydin et al. World J Gastroenterol. .

Abstract

Aim: To investigate in vitro effects of propofol, midazolam and dexmedetomidine, which are commonly used anaesthesic or sedatives, on spontaneous contractions of the ileum both in normal rats and those exposed to hyperdynamic peritonitis.

Methods: Spontaneous contractions of isolated ileum muscle segments from sham operated rats and those exposed to peritonitis, were studied in vitro. The amplitude and the frequency of spontaneous contractions of ileum muscle segments were studied after adding dexmedetomidine, propofol, and midazolam to the organ bath in a cumulative manner.

Results: Both amplitude (85.2 +/- 6.6 vs 47.4 +/- 7.1) and frequency (32.8 +/- 4.6 vs 20.2 +/- 3.9) of spontaneous contractions in ileum smooth muscle segments were decreased significantly in the peritonitis group compared to the control group (P < 0.05). Dexmedetomidine significantly increased the amplitude of spontaneous contractions (85.2 +/- 6.6 vs 152.0 +/- 5.4, P < 0.05) whereas, propofol (85.2 +/- 6.6 vs 49.6 +/- 4.8, P < 0.05) and midazolam (85.2 +/- 6.6 vs 39.2 +/- 4.5, P < 0.05) decreased it in both control and peritonitis groups. The frequency of spontaneous contractions were significantly decreased by propofol in both control (32.8 +/- 4.6 vs 18.2 +/- 3.4, P < 0.05) and peritonitis groups 20.2 +/- 3.9 vs 11.6 +/- 3.2, P < 0.05). Dexmedetomidine and midazolam did not cause significant changes in the number of spontaneous contractions in both control and the peritonitis groups (P > 0.05).

Conclusion: Propofol, midazolam and dexmedetomidine have various in vitro effects on spontaneous contractions of the rat ileum. While dexmedetomidine augments the spontaneous contraction of the rat ileum, propofol attenuates it. However, the effects of these compounds were parallel in both control and peritonitis groups.

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Figures

Figure 1
Figure 1
KCl (80 mmol/L) induced contractions of isolated ileum muscle segments in control and peritonitis groups. No statistical difference was observed between groups (P > 0.05).
Figure 2
Figure 2
Changes in the spontaneous contractions of the isolated ileum muscle segments. A: Amplitudes were calculated as a percentage of the contraction induced by KCl (80 mmol/L) from both control and peritonitis groups; B: Frequencies were expressed as the number of contractions for 10 min from both control and peritonitis groups. (aP < 0.05 vs control group; analysis of variance followed by Newman-Keuls test).
Figure 3
Figure 3
Amplitudes of the contractions induced by anaesthetic agents. A: Control group; B: Peritonitis group; both were calculated as the percentage of the initial contractions. (aP < 0.05 vs initial contractions, cP < 0.05 vs propofol and midazolam; analysis of variance followed by Newman-Keuls test).
Figure 4
Figure 4
Changes in the frequency of spontaneous contractions. A: Control group; B: Peritonitis group. Both were expressed as the number of contractions for 10 min. (aP < 0.05 vs initial contractions; analysis of variance followed by Newman-Keuls test).
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