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. 1977 Mar 29;202(1):45-54.
doi: 10.1007/BF00496767.

Cytochemical localiztion of nucleoside diphosphatase and thiamine pyrophosphatase activities in the rabbit corneal endothelium

S Yokota, W K Waller

Cytochemical localiztion of nucleoside diphosphatase and thiamine pyrophosphatase activities in the rabbit corneal endothelium

S Yokota et al. Albrecht Von Graefes Arch Klin Exp Ophthalmol. .

Abstract

Localization of NDPase and TPPase activities in the rabbit corneal endothelium was investigated by cytochemical methods. After incubation at both pH 7.0 and 8.0, the NDPase activity was detected in cisternae of ER, nuclear envelope, and Golgi saccules, while the TPPase activity was demonstrated only in Golgi saccules. The reaction products of both enzymes were decreased when incubated at pH 8.0. The NDPase activity was inhibited by uranyl nitrate but not by sodium fluoride, while the TPPase was inhibited by fluoride but not by uranium.

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