doi: 10.1016/j.mimet.2009.03.009.
Epub 2009 Mar 31.
One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae
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- PMID: 19341768
- DOI: 10.1016/j.mimet.2009.03.009
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One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae
J Microbiol Methods.
2009 Jun.
Abstract
One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction.
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